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assays rac activation assay biochem kit cytoskeleton cat  (Cytoskeleton Inc)


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    Cytoskeleton Inc assays rac activation assay biochem kit cytoskeleton cat
    Assays Rac Activation Assay Biochem Kit Cytoskeleton Cat, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/assays+rac+activation+assay+biochem+kit+cytoskeleton+cat/Rac1+Pulldown+Activation+Assay+Kit/pm33657382-186-223-229
    Average 95 stars, based on 271 article reviews
    assays rac activation assay biochem kit cytoskeleton cat - by Bioz Stars, 2026-10
    95/100 stars

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    Virus:

    Article Title: Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ILK Genetex Cat#GTX10169; RRID:AB_1950586 Rabbit monoclonal anti-b-arrestin1 Abcam Cat#32099; RRID:AB_722896 Rabbit polyclonal anti-b-arrestin1 LSBio Cat#LSC156512 Rabbit polyclonal anti-bPix Cell Signaling Technology Cat#4515 Mouse monoclonal anti-bPix (H-3) Santa Cruz Biotechnology Cat#sc-393184 Rabbit polyclonal anti-phospho-PAK1 (Thr423)/PAK2(Thr402) Cell Signaling Technology Cat#2601 Rabbit polyclonal anti-PAK1 Cell Signaling Technology Cat#2602 Rabbit monoclonal anti-Rac3 Abcam Cat#ab129062; RRID:AB_11143779 Mouse monoclonal anti-aTubulin Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Mouse monoclonal anti-AU5 tag Abcam Cat#ab24576; RRID:AB_448153 Mouse monoclonal anti-MMP14 Santa Cruz Biotechnology Cat#sc-12366; RRID:AB_673759 Rabbit polyclonal anti-phospho-Cortactin (Tyr421) Abcam Cat#47768; RRID:AB_869231 Rabbit polyclonal anti-Cortactin (H222) Cell Signaling Technology Cat#3503; RRID:AB_2115160 Mouse monoclonal anti-Cortactin [4F11] Abcam Cat#ab33333; RRID:AB_731713 Rabbit monoclonal anti-phospho-Cofilin (Ser3) Cell Signaling Technology Cat#3313; RRID:AB_2080597 Rabbit monoclonal anti-Cofilin (D3F9) XP Cell Signaling Technology Cat#5175; RRID:AB_10622000 Mouse monoclonal b-arrestin2 Santa Cruz Biotechnology Cat#sc-13140 Rabbit polyclonal anti-FLAG Cell Signaling Technology Cat#2638 Rabbit polyclonal anti-ETAR Thermo Fisher Scientific Cat#PA3-065 Mouse monoclonal anti-Threonine Immunological Sciences Cat#MAB-80131 Mouse monoclonal anti-Tyrosine Immunological Sciences Cat#MAB-94223 Rabbit polyclonal anti-Paxillin GeneTex Cat#GTX125891 Bacterial and virus strains BL21(DE3) Chemically Competent E. coli Thermo Fisher scientific C600003 Chemicals, peptides, and recombinant proteins Fibronectin human foreskin Sigma-Aldrich Cat#F2518-5MG Collagen Type I rat tail BD biosciences Cat#MA01730 Collagen type I From Bovine Skin, Fluorescein Conjugate Invitrogen Cat#D12060 BQ-788 sodium salt Bachem Cat#4058874 N-cis-2,6-Dimethylpiperidinocarbonylb-tBu-Ala-D-Trp(1-methoxycarbonyl)-D-Nle-OH Cultrex Basement Membrane Matrix Trevigen Cat#3432-001-01 Ilomastat Millipore Cat#GM6001 Ambrisentan Sigma-Aldrich Cat#SML2104 Critical commercial assays Rac Activation Assay Biochem Kit Cytoskeleton CatBK035 Cultrex 3D Spheroid Cell Invasion Assay Trevigen Cat#3500-096-K Experimental models: cell lines Human cell lines: SKOV3 American Type Culture Collection (ATCC) ATCC HTB-77; RRID:CVCL_0532 (Continued on next page) Cell Reports 34, 108800, March 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human cell lines: OVCAR3 American Type Culture Collection (ATCC) ATCC HTB-161; RRID:CVCL_0465 Human cell lines: CAOV3 American Type Culture Collection (ATCC) ATCC HTB-75; RRID:CVCL_0201 Human primary mesothelial cell Zen-Bio Cat#MSO-1; Cat#DME-F Experimental models: organisms/strains Mouse: NOD/SCID 4-5 weeks (female) Charles River laboratories N/A Oligonucleotides Primers for ILK: Eurofins Genomics N/A Forward: 50-GAGAGCGGGCAGAGAAGATG-30 Reverse: 50-GAGTGTTTGTTCAGGGTTCCATTT-30 Primers for bPIX(PIXB): Eurofins Genomics N/A Forward: 50-AGCTCGAGAGACACATGGAGGATT-30 Reverse: 50-TCTGCAGCTCAAGCTCTTTCCTCT-30 Primers for Rac3 (RAC3): Eurofins Genomics N/A Forward: 50-GACGACAAGGACACC-30 Reverse: 50-CCTCGTCAAACAGTC-30 Primers for b-arr1(ARRB1): Invitrogen Cat#824370 Forward: 50-GAGCACGCTCTTACCCTTTCAC-30 Reverse: 50-TCTCTGGGGCATACTGAACC-30 Primers for Cyclophilin A: Eurofins Genomics N/A Forward: 50- TTCATCTGCACTGCCAAGAC-30 Reverse: 50-TCGAGTTGTCCACAGTCAGC-30 siRNA targeting sequence: ILK: (13.2) IDT integrated DNA technologies N/A 50-AUGAUUGUGCCUAUCCUUGAGAAGA-30 30-UGUACUAACACGGAUAGGAACUCUUCU-50 SiRNA targeting sequence: bPIX (PIXB): (13.1) IDT integrated DNA technologies N/A 50-GAAAGAUAUCUUCUACUCUUCCCAA-30 30-UACUUUCUAUAGAAGAUGAGAAGGGUU-50 SiRNA targeting sequence: Rac3 (RAC3): (13.1) IDT integrated DNA technologies N/A 50-GACGUCUUUCUGAUCUGCUUCUCTC-30 30-GACUGCAGAAAGACUAGACGAAGAGAG-50 SiRNA targeting sequence: MT1-MMP: (13.1), (13.2) and (13.3) IDT integrated DNA technologies N/A 50-GGCAACAUAAUGAAAUCACUUUCTG-30 30-UACCGUUGUAUUACUUUAGUGAAAGAC-50 50-GCCAUGAAGUCUUCACUUACUUCTA-30 30-GUCGCUACUUCAGAAGUGAAUGAAGAU-50 50-UGUGGCUAAAAGGAAUCUAAUCUTG-30 30-AAAGACCGAUUUUCCUUAGAUUAGAAC-50 SiRNA targeting sequence: TKS5 (SH3PXD2A): (13.2) IDT integrated DNA technologies N/A 50-CAAGCACUGAACACCGUCAACCAGA-30 30-AGGUUCGUGACUUGUGGCAGUUGGUCU-50 SiRNA targeting sequence: b-arr1(ARRB1) Dharmacon Cat#L-011971-00 SMARTpool SiRNA targeting sequence: ETAR (EDNRA) Dharmacon Cat#L-005485-00 SMARTpool (Continued on next page) e2 Cell Reports 34, 108800, March 2, 2021

    Recombinant:

    Article Title: Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ILK Genetex Cat#GTX10169; RRID:AB_1950586 Rabbit monoclonal anti-b-arrestin1 Abcam Cat#32099; RRID:AB_722896 Rabbit polyclonal anti-b-arrestin1 LSBio Cat#LSC156512 Rabbit polyclonal anti-bPix Cell Signaling Technology Cat#4515 Mouse monoclonal anti-bPix (H-3) Santa Cruz Biotechnology Cat#sc-393184 Rabbit polyclonal anti-phospho-PAK1 (Thr423)/PAK2(Thr402) Cell Signaling Technology Cat#2601 Rabbit polyclonal anti-PAK1 Cell Signaling Technology Cat#2602 Rabbit monoclonal anti-Rac3 Abcam Cat#ab129062; RRID:AB_11143779 Mouse monoclonal anti-aTubulin Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Mouse monoclonal anti-AU5 tag Abcam Cat#ab24576; RRID:AB_448153 Mouse monoclonal anti-MMP14 Santa Cruz Biotechnology Cat#sc-12366; RRID:AB_673759 Rabbit polyclonal anti-phospho-Cortactin (Tyr421) Abcam Cat#47768; RRID:AB_869231 Rabbit polyclonal anti-Cortactin (H222) Cell Signaling Technology Cat#3503; RRID:AB_2115160 Mouse monoclonal anti-Cortactin [4F11] Abcam Cat#ab33333; RRID:AB_731713 Rabbit monoclonal anti-phospho-Cofilin (Ser3) Cell Signaling Technology Cat#3313; RRID:AB_2080597 Rabbit monoclonal anti-Cofilin (D3F9) XP Cell Signaling Technology Cat#5175; RRID:AB_10622000 Mouse monoclonal b-arrestin2 Santa Cruz Biotechnology Cat#sc-13140 Rabbit polyclonal anti-FLAG Cell Signaling Technology Cat#2638 Rabbit polyclonal anti-ETAR Thermo Fisher Scientific Cat#PA3-065 Mouse monoclonal anti-Threonine Immunological Sciences Cat#MAB-80131 Mouse monoclonal anti-Tyrosine Immunological Sciences Cat#MAB-94223 Rabbit polyclonal anti-Paxillin GeneTex Cat#GTX125891 Bacterial and virus strains BL21(DE3) Chemically Competent E. coli Thermo Fisher scientific C600003 Chemicals, peptides, and recombinant proteins Fibronectin human foreskin Sigma-Aldrich Cat#F2518-5MG Collagen Type I rat tail BD biosciences Cat#MA01730 Collagen type I From Bovine Skin, Fluorescein Conjugate Invitrogen Cat#D12060 BQ-788 sodium salt Bachem Cat#4058874 N-cis-2,6-Dimethylpiperidinocarbonylb-tBu-Ala-D-Trp(1-methoxycarbonyl)-D-Nle-OH Cultrex Basement Membrane Matrix Trevigen Cat#3432-001-01 Ilomastat Millipore Cat#GM6001 Ambrisentan Sigma-Aldrich Cat#SML2104 Critical commercial assays Rac Activation Assay Biochem Kit Cytoskeleton CatBK035 Cultrex 3D Spheroid Cell Invasion Assay Trevigen Cat#3500-096-K Experimental models: cell lines Human cell lines: SKOV3 American Type Culture Collection (ATCC) ATCC HTB-77; RRID:CVCL_0532 (Continued on next page) Cell Reports 34, 108800, March 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human cell lines: OVCAR3 American Type Culture Collection (ATCC) ATCC HTB-161; RRID:CVCL_0465 Human cell lines: CAOV3 American Type Culture Collection (ATCC) ATCC HTB-75; RRID:CVCL_0201 Human primary mesothelial cell Zen-Bio Cat#MSO-1; Cat#DME-F Experimental models: organisms/strains Mouse: NOD/SCID 4-5 weeks (female) Charles River laboratories N/A Oligonucleotides Primers for ILK: Eurofins Genomics N/A Forward: 50-GAGAGCGGGCAGAGAAGATG-30 Reverse: 50-GAGTGTTTGTTCAGGGTTCCATTT-30 Primers for bPIX(PIXB): Eurofins Genomics N/A Forward: 50-AGCTCGAGAGACACATGGAGGATT-30 Reverse: 50-TCTGCAGCTCAAGCTCTTTCCTCT-30 Primers for Rac3 (RAC3): Eurofins Genomics N/A Forward: 50-GACGACAAGGACACC-30 Reverse: 50-CCTCGTCAAACAGTC-30 Primers for b-arr1(ARRB1): Invitrogen Cat#824370 Forward: 50-GAGCACGCTCTTACCCTTTCAC-30 Reverse: 50-TCTCTGGGGCATACTGAACC-30 Primers for Cyclophilin A: Eurofins Genomics N/A Forward: 50- TTCATCTGCACTGCCAAGAC-30 Reverse: 50-TCGAGTTGTCCACAGTCAGC-30 siRNA targeting sequence: ILK: (13.2) IDT integrated DNA technologies N/A 50-AUGAUUGUGCCUAUCCUUGAGAAGA-30 30-UGUACUAACACGGAUAGGAACUCUUCU-50 SiRNA targeting sequence: bPIX (PIXB): (13.1) IDT integrated DNA technologies N/A 50-GAAAGAUAUCUUCUACUCUUCCCAA-30 30-UACUUUCUAUAGAAGAUGAGAAGGGUU-50 SiRNA targeting sequence: Rac3 (RAC3): (13.1) IDT integrated DNA technologies N/A 50-GACGUCUUUCUGAUCUGCUUCUCTC-30 30-GACUGCAGAAAGACUAGACGAAGAGAG-50 SiRNA targeting sequence: MT1-MMP: (13.1), (13.2) and (13.3) IDT integrated DNA technologies N/A 50-GGCAACAUAAUGAAAUCACUUUCTG-30 30-UACCGUUGUAUUACUUUAGUGAAAGAC-50 50-GCCAUGAAGUCUUCACUUACUUCTA-30 30-GUCGCUACUUCAGAAGUGAAUGAAGAU-50 50-UGUGGCUAAAAGGAAUCUAAUCUTG-30 30-AAAGACCGAUUUUCCUUAGAUUAGAAC-50 SiRNA targeting sequence: TKS5 (SH3PXD2A): (13.2) IDT integrated DNA technologies N/A 50-CAAGCACUGAACACCGUCAACCAGA-30 30-AGGUUCGUGACUUGUGGCAGUUGGUCU-50 SiRNA targeting sequence: b-arr1(ARRB1) Dharmacon Cat#L-011971-00 SMARTpool SiRNA targeting sequence: ETAR (EDNRA) Dharmacon Cat#L-005485-00 SMARTpool (Continued on next page) e2 Cell Reports 34, 108800, March 2, 2021

    Membrane:

    Article Title: Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ILK Genetex Cat#GTX10169; RRID:AB_1950586 Rabbit monoclonal anti-b-arrestin1 Abcam Cat#32099; RRID:AB_722896 Rabbit polyclonal anti-b-arrestin1 LSBio Cat#LSC156512 Rabbit polyclonal anti-bPix Cell Signaling Technology Cat#4515 Mouse monoclonal anti-bPix (H-3) Santa Cruz Biotechnology Cat#sc-393184 Rabbit polyclonal anti-phospho-PAK1 (Thr423)/PAK2(Thr402) Cell Signaling Technology Cat#2601 Rabbit polyclonal anti-PAK1 Cell Signaling Technology Cat#2602 Rabbit monoclonal anti-Rac3 Abcam Cat#ab129062; RRID:AB_11143779 Mouse monoclonal anti-aTubulin Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Mouse monoclonal anti-AU5 tag Abcam Cat#ab24576; RRID:AB_448153 Mouse monoclonal anti-MMP14 Santa Cruz Biotechnology Cat#sc-12366; RRID:AB_673759 Rabbit polyclonal anti-phospho-Cortactin (Tyr421) Abcam Cat#47768; RRID:AB_869231 Rabbit polyclonal anti-Cortactin (H222) Cell Signaling Technology Cat#3503; RRID:AB_2115160 Mouse monoclonal anti-Cortactin [4F11] Abcam Cat#ab33333; RRID:AB_731713 Rabbit monoclonal anti-phospho-Cofilin (Ser3) Cell Signaling Technology Cat#3313; RRID:AB_2080597 Rabbit monoclonal anti-Cofilin (D3F9) XP Cell Signaling Technology Cat#5175; RRID:AB_10622000 Mouse monoclonal b-arrestin2 Santa Cruz Biotechnology Cat#sc-13140 Rabbit polyclonal anti-FLAG Cell Signaling Technology Cat#2638 Rabbit polyclonal anti-ETAR Thermo Fisher Scientific Cat#PA3-065 Mouse monoclonal anti-Threonine Immunological Sciences Cat#MAB-80131 Mouse monoclonal anti-Tyrosine Immunological Sciences Cat#MAB-94223 Rabbit polyclonal anti-Paxillin GeneTex Cat#GTX125891 Bacterial and virus strains BL21(DE3) Chemically Competent E. coli Thermo Fisher scientific C600003 Chemicals, peptides, and recombinant proteins Fibronectin human foreskin Sigma-Aldrich Cat#F2518-5MG Collagen Type I rat tail BD biosciences Cat#MA01730 Collagen type I From Bovine Skin, Fluorescein Conjugate Invitrogen Cat#D12060 BQ-788 sodium salt Bachem Cat#4058874 N-cis-2,6-Dimethylpiperidinocarbonylb-tBu-Ala-D-Trp(1-methoxycarbonyl)-D-Nle-OH Cultrex Basement Membrane Matrix Trevigen Cat#3432-001-01 Ilomastat Millipore Cat#GM6001 Ambrisentan Sigma-Aldrich Cat#SML2104 Critical commercial assays Rac Activation Assay Biochem Kit Cytoskeleton CatBK035 Cultrex 3D Spheroid Cell Invasion Assay Trevigen Cat#3500-096-K Experimental models: cell lines Human cell lines: SKOV3 American Type Culture Collection (ATCC) ATCC HTB-77; RRID:CVCL_0532 (Continued on next page) Cell Reports 34, 108800, March 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human cell lines: OVCAR3 American Type Culture Collection (ATCC) ATCC HTB-161; RRID:CVCL_0465 Human cell lines: CAOV3 American Type Culture Collection (ATCC) ATCC HTB-75; RRID:CVCL_0201 Human primary mesothelial cell Zen-Bio Cat#MSO-1; Cat#DME-F Experimental models: organisms/strains Mouse: NOD/SCID 4-5 weeks (female) Charles River laboratories N/A Oligonucleotides Primers for ILK: Eurofins Genomics N/A Forward: 50-GAGAGCGGGCAGAGAAGATG-30 Reverse: 50-GAGTGTTTGTTCAGGGTTCCATTT-30 Primers for bPIX(PIXB): Eurofins Genomics N/A Forward: 50-AGCTCGAGAGACACATGGAGGATT-30 Reverse: 50-TCTGCAGCTCAAGCTCTTTCCTCT-30 Primers for Rac3 (RAC3): Eurofins Genomics N/A Forward: 50-GACGACAAGGACACC-30 Reverse: 50-CCTCGTCAAACAGTC-30 Primers for b-arr1(ARRB1): Invitrogen Cat#824370 Forward: 50-GAGCACGCTCTTACCCTTTCAC-30 Reverse: 50-TCTCTGGGGCATACTGAACC-30 Primers for Cyclophilin A: Eurofins Genomics N/A Forward: 50- TTCATCTGCACTGCCAAGAC-30 Reverse: 50-TCGAGTTGTCCACAGTCAGC-30 siRNA targeting sequence: ILK: (13.2) IDT integrated DNA technologies N/A 50-AUGAUUGUGCCUAUCCUUGAGAAGA-30 30-UGUACUAACACGGAUAGGAACUCUUCU-50 SiRNA targeting sequence: bPIX (PIXB): (13.1) IDT integrated DNA technologies N/A 50-GAAAGAUAUCUUCUACUCUUCCCAA-30 30-UACUUUCUAUAGAAGAUGAGAAGGGUU-50 SiRNA targeting sequence: Rac3 (RAC3): (13.1) IDT integrated DNA technologies N/A 50-GACGUCUUUCUGAUCUGCUUCUCTC-30 30-GACUGCAGAAAGACUAGACGAAGAGAG-50 SiRNA targeting sequence: MT1-MMP: (13.1), (13.2) and (13.3) IDT integrated DNA technologies N/A 50-GGCAACAUAAUGAAAUCACUUUCTG-30 30-UACCGUUGUAUUACUUUAGUGAAAGAC-50 50-GCCAUGAAGUCUUCACUUACUUCTA-30 30-GUCGCUACUUCAGAAGUGAAUGAAGAU-50 50-UGUGGCUAAAAGGAAUCUAAUCUTG-30 30-AAAGACCGAUUUUCCUUAGAUUAGAAC-50 SiRNA targeting sequence: TKS5 (SH3PXD2A): (13.2) IDT integrated DNA technologies N/A 50-CAAGCACUGAACACCGUCAACCAGA-30 30-AGGUUCGUGACUUGUGGCAGUUGGUCU-50 SiRNA targeting sequence: b-arr1(ARRB1) Dharmacon Cat#L-011971-00 SMARTpool SiRNA targeting sequence: ETAR (EDNRA) Dharmacon Cat#L-005485-00 SMARTpool (Continued on next page) e2 Cell Reports 34, 108800, March 2, 2021

    Activation Assay:

    Article Title: Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ILK Genetex Cat#GTX10169; RRID:AB_1950586 Rabbit monoclonal anti-b-arrestin1 Abcam Cat#32099; RRID:AB_722896 Rabbit polyclonal anti-b-arrestin1 LSBio Cat#LSC156512 Rabbit polyclonal anti-bPix Cell Signaling Technology Cat#4515 Mouse monoclonal anti-bPix (H-3) Santa Cruz Biotechnology Cat#sc-393184 Rabbit polyclonal anti-phospho-PAK1 (Thr423)/PAK2(Thr402) Cell Signaling Technology Cat#2601 Rabbit polyclonal anti-PAK1 Cell Signaling Technology Cat#2602 Rabbit monoclonal anti-Rac3 Abcam Cat#ab129062; RRID:AB_11143779 Mouse monoclonal anti-aTubulin Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Mouse monoclonal anti-AU5 tag Abcam Cat#ab24576; RRID:AB_448153 Mouse monoclonal anti-MMP14 Santa Cruz Biotechnology Cat#sc-12366; RRID:AB_673759 Rabbit polyclonal anti-phospho-Cortactin (Tyr421) Abcam Cat#47768; RRID:AB_869231 Rabbit polyclonal anti-Cortactin (H222) Cell Signaling Technology Cat#3503; RRID:AB_2115160 Mouse monoclonal anti-Cortactin [4F11] Abcam Cat#ab33333; RRID:AB_731713 Rabbit monoclonal anti-phospho-Cofilin (Ser3) Cell Signaling Technology Cat#3313; RRID:AB_2080597 Rabbit monoclonal anti-Cofilin (D3F9) XP Cell Signaling Technology Cat#5175; RRID:AB_10622000 Mouse monoclonal b-arrestin2 Santa Cruz Biotechnology Cat#sc-13140 Rabbit polyclonal anti-FLAG Cell Signaling Technology Cat#2638 Rabbit polyclonal anti-ETAR Thermo Fisher Scientific Cat#PA3-065 Mouse monoclonal anti-Threonine Immunological Sciences Cat#MAB-80131 Mouse monoclonal anti-Tyrosine Immunological Sciences Cat#MAB-94223 Rabbit polyclonal anti-Paxillin GeneTex Cat#GTX125891 Bacterial and virus strains BL21(DE3) Chemically Competent E. coli Thermo Fisher scientific C600003 Chemicals, peptides, and recombinant proteins Fibronectin human foreskin Sigma-Aldrich Cat#F2518-5MG Collagen Type I rat tail BD biosciences Cat#MA01730 Collagen type I From Bovine Skin, Fluorescein Conjugate Invitrogen Cat#D12060 BQ-788 sodium salt Bachem Cat#4058874 N-cis-2,6-Dimethylpiperidinocarbonylb-tBu-Ala-D-Trp(1-methoxycarbonyl)-D-Nle-OH Cultrex Basement Membrane Matrix Trevigen Cat#3432-001-01 Ilomastat Millipore Cat#GM6001 Ambrisentan Sigma-Aldrich Cat#SML2104 Critical commercial assays Rac Activation Assay Biochem Kit Cytoskeleton CatBK035 Cultrex 3D Spheroid Cell Invasion Assay Trevigen Cat#3500-096-K Experimental models: cell lines Human cell lines: SKOV3 American Type Culture Collection (ATCC) ATCC HTB-77; RRID:CVCL_0532 (Continued on next page) Cell Reports 34, 108800, March 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human cell lines: OVCAR3 American Type Culture Collection (ATCC) ATCC HTB-161; RRID:CVCL_0465 Human cell lines: CAOV3 American Type Culture Collection (ATCC) ATCC HTB-75; RRID:CVCL_0201 Human primary mesothelial cell Zen-Bio Cat#MSO-1; Cat#DME-F Experimental models: organisms/strains Mouse: NOD/SCID 4-5 weeks (female) Charles River laboratories N/A Oligonucleotides Primers for ILK: Eurofins Genomics N/A Forward: 50-GAGAGCGGGCAGAGAAGATG-30 Reverse: 50-GAGTGTTTGTTCAGGGTTCCATTT-30 Primers for bPIX(PIXB): Eurofins Genomics N/A Forward: 50-AGCTCGAGAGACACATGGAGGATT-30 Reverse: 50-TCTGCAGCTCAAGCTCTTTCCTCT-30 Primers for Rac3 (RAC3): Eurofins Genomics N/A Forward: 50-GACGACAAGGACACC-30 Reverse: 50-CCTCGTCAAACAGTC-30 Primers for b-arr1(ARRB1): Invitrogen Cat#824370 Forward: 50-GAGCACGCTCTTACCCTTTCAC-30 Reverse: 50-TCTCTGGGGCATACTGAACC-30 Primers for Cyclophilin A: Eurofins Genomics N/A Forward: 50- TTCATCTGCACTGCCAAGAC-30 Reverse: 50-TCGAGTTGTCCACAGTCAGC-30 siRNA targeting sequence: ILK: (13.2) IDT integrated DNA technologies N/A 50-AUGAUUGUGCCUAUCCUUGAGAAGA-30 30-UGUACUAACACGGAUAGGAACUCUUCU-50 SiRNA targeting sequence: bPIX (PIXB): (13.1) IDT integrated DNA technologies N/A 50-GAAAGAUAUCUUCUACUCUUCCCAA-30 30-UACUUUCUAUAGAAGAUGAGAAGGGUU-50 SiRNA targeting sequence: Rac3 (RAC3): (13.1) IDT integrated DNA technologies N/A 50-GACGUCUUUCUGAUCUGCUUCUCTC-30 30-GACUGCAGAAAGACUAGACGAAGAGAG-50 SiRNA targeting sequence: MT1-MMP: (13.1), (13.2) and (13.3) IDT integrated DNA technologies N/A 50-GGCAACAUAAUGAAAUCACUUUCTG-30 30-UACCGUUGUAUUACUUUAGUGAAAGAC-50 50-GCCAUGAAGUCUUCACUUACUUCTA-30 30-GUCGCUACUUCAGAAGUGAAUGAAGAU-50 50-UGUGGCUAAAAGGAAUCUAAUCUTG-30 30-AAAGACCGAUUUUCCUUAGAUUAGAAC-50 SiRNA targeting sequence: TKS5 (SH3PXD2A): (13.2) IDT integrated DNA technologies N/A 50-CAAGCACUGAACACCGUCAACCAGA-30 30-AGGUUCGUGACUUGUGGCAGUUGGUCU-50 SiRNA targeting sequence: b-arr1(ARRB1) Dharmacon Cat#L-011971-00 SMARTpool SiRNA targeting sequence: ETAR (EDNRA) Dharmacon Cat#L-005485-00 SMARTpool (Continued on next page) e2 Cell Reports 34, 108800, March 2, 2021

    Invasion Assay:

    Article Title: Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ILK Genetex Cat#GTX10169; RRID:AB_1950586 Rabbit monoclonal anti-b-arrestin1 Abcam Cat#32099; RRID:AB_722896 Rabbit polyclonal anti-b-arrestin1 LSBio Cat#LSC156512 Rabbit polyclonal anti-bPix Cell Signaling Technology Cat#4515 Mouse monoclonal anti-bPix (H-3) Santa Cruz Biotechnology Cat#sc-393184 Rabbit polyclonal anti-phospho-PAK1 (Thr423)/PAK2(Thr402) Cell Signaling Technology Cat#2601 Rabbit polyclonal anti-PAK1 Cell Signaling Technology Cat#2602 Rabbit monoclonal anti-Rac3 Abcam Cat#ab129062; RRID:AB_11143779 Mouse monoclonal anti-aTubulin Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Mouse monoclonal anti-AU5 tag Abcam Cat#ab24576; RRID:AB_448153 Mouse monoclonal anti-MMP14 Santa Cruz Biotechnology Cat#sc-12366; RRID:AB_673759 Rabbit polyclonal anti-phospho-Cortactin (Tyr421) Abcam Cat#47768; RRID:AB_869231 Rabbit polyclonal anti-Cortactin (H222) Cell Signaling Technology Cat#3503; RRID:AB_2115160 Mouse monoclonal anti-Cortactin [4F11] Abcam Cat#ab33333; RRID:AB_731713 Rabbit monoclonal anti-phospho-Cofilin (Ser3) Cell Signaling Technology Cat#3313; RRID:AB_2080597 Rabbit monoclonal anti-Cofilin (D3F9) XP Cell Signaling Technology Cat#5175; RRID:AB_10622000 Mouse monoclonal b-arrestin2 Santa Cruz Biotechnology Cat#sc-13140 Rabbit polyclonal anti-FLAG Cell Signaling Technology Cat#2638 Rabbit polyclonal anti-ETAR Thermo Fisher Scientific Cat#PA3-065 Mouse monoclonal anti-Threonine Immunological Sciences Cat#MAB-80131 Mouse monoclonal anti-Tyrosine Immunological Sciences Cat#MAB-94223 Rabbit polyclonal anti-Paxillin GeneTex Cat#GTX125891 Bacterial and virus strains BL21(DE3) Chemically Competent E. coli Thermo Fisher scientific C600003 Chemicals, peptides, and recombinant proteins Fibronectin human foreskin Sigma-Aldrich Cat#F2518-5MG Collagen Type I rat tail BD biosciences Cat#MA01730 Collagen type I From Bovine Skin, Fluorescein Conjugate Invitrogen Cat#D12060 BQ-788 sodium salt Bachem Cat#4058874 N-cis-2,6-Dimethylpiperidinocarbonylb-tBu-Ala-D-Trp(1-methoxycarbonyl)-D-Nle-OH Cultrex Basement Membrane Matrix Trevigen Cat#3432-001-01 Ilomastat Millipore Cat#GM6001 Ambrisentan Sigma-Aldrich Cat#SML2104 Critical commercial assays Rac Activation Assay Biochem Kit Cytoskeleton CatBK035 Cultrex 3D Spheroid Cell Invasion Assay Trevigen Cat#3500-096-K Experimental models: cell lines Human cell lines: SKOV3 American Type Culture Collection (ATCC) ATCC HTB-77; RRID:CVCL_0532 (Continued on next page) Cell Reports 34, 108800, March 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human cell lines: OVCAR3 American Type Culture Collection (ATCC) ATCC HTB-161; RRID:CVCL_0465 Human cell lines: CAOV3 American Type Culture Collection (ATCC) ATCC HTB-75; RRID:CVCL_0201 Human primary mesothelial cell Zen-Bio Cat#MSO-1; Cat#DME-F Experimental models: organisms/strains Mouse: NOD/SCID 4-5 weeks (female) Charles River laboratories N/A Oligonucleotides Primers for ILK: Eurofins Genomics N/A Forward: 50-GAGAGCGGGCAGAGAAGATG-30 Reverse: 50-GAGTGTTTGTTCAGGGTTCCATTT-30 Primers for bPIX(PIXB): Eurofins Genomics N/A Forward: 50-AGCTCGAGAGACACATGGAGGATT-30 Reverse: 50-TCTGCAGCTCAAGCTCTTTCCTCT-30 Primers for Rac3 (RAC3): Eurofins Genomics N/A Forward: 50-GACGACAAGGACACC-30 Reverse: 50-CCTCGTCAAACAGTC-30 Primers for b-arr1(ARRB1): Invitrogen Cat#824370 Forward: 50-GAGCACGCTCTTACCCTTTCAC-30 Reverse: 50-TCTCTGGGGCATACTGAACC-30 Primers for Cyclophilin A: Eurofins Genomics N/A Forward: 50- TTCATCTGCACTGCCAAGAC-30 Reverse: 50-TCGAGTTGTCCACAGTCAGC-30 siRNA targeting sequence: ILK: (13.2) IDT integrated DNA technologies N/A 50-AUGAUUGUGCCUAUCCUUGAGAAGA-30 30-UGUACUAACACGGAUAGGAACUCUUCU-50 SiRNA targeting sequence: bPIX (PIXB): (13.1) IDT integrated DNA technologies N/A 50-GAAAGAUAUCUUCUACUCUUCCCAA-30 30-UACUUUCUAUAGAAGAUGAGAAGGGUU-50 SiRNA targeting sequence: Rac3 (RAC3): (13.1) IDT integrated DNA technologies N/A 50-GACGUCUUUCUGAUCUGCUUCUCTC-30 30-GACUGCAGAAAGACUAGACGAAGAGAG-50 SiRNA targeting sequence: MT1-MMP: (13.1), (13.2) and (13.3) IDT integrated DNA technologies N/A 50-GGCAACAUAAUGAAAUCACUUUCTG-30 30-UACCGUUGUAUUACUUUAGUGAAAGAC-50 50-GCCAUGAAGUCUUCACUUACUUCTA-30 30-GUCGCUACUUCAGAAGUGAAUGAAGAU-50 50-UGUGGCUAAAAGGAAUCUAAUCUTG-30 30-AAAGACCGAUUUUCCUUAGAUUAGAAC-50 SiRNA targeting sequence: TKS5 (SH3PXD2A): (13.2) IDT integrated DNA technologies N/A 50-CAAGCACUGAACACCGUCAACCAGA-30 30-AGGUUCGUGACUUGUGGCAGUUGGUCU-50 SiRNA targeting sequence: b-arr1(ARRB1) Dharmacon Cat#L-011971-00 SMARTpool SiRNA targeting sequence: ETAR (EDNRA) Dharmacon Cat#L-005485-00 SMARTpool (Continued on next page) e2 Cell Reports 34, 108800, March 2, 2021



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